We follow these rules specifically. The process is very easy, but make sure you follow the rules and you'll be performing from memory before you know it!!! Hands must be washed before putting on gloves. Surfaces must be wiped down with sodium hypochlorite (bleach water) before and after testing. Label tray with site number, collection time/date, processing time/date, processor's initials. Retrieve sample and Colilert from refrigerator. Aseptically (just means free of pathogenic microorganisms) add one packet of reagent to the sample. Re-cap bottle and shake until reagent is mostly dissolved. (If sample "flashes blue" it contains excessive chlorine and is invalid for analysis) Pour into Quanti-Tray. Place tray in rubber insert for Quanti-Tray sealer machine. The sealer works like a laminator and seals the backing onto the tray. Feed tray into Quanti-Tray sealer. Place tray into incubator and incubate at 35.0 degrees Celsius for 24 hours . After incubation, the tray is